Journal: Nature Communications

Article Title: TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory network

doi: 10.1038/s41467-026-72077-5

Figure Lengend Snippet: a Scheme for the generation of an inducible β-cell-specific TBL1X and TBL1XR1 knockout. b Random fed blood glucose levels over time in iTBL/RβKO ( n = 9) and control ( n = 9) mice on HFD. c Total pancreas insulin content normalized to protein levels in iTBL/RβKO and control mice 4 and 11 weeks after tamoxifen administration. n = 3 for 4w control and iTBL/RβKO mice, n = 6 for 11w control mice, n = 4 for 11w iTBL/RβKO mice. d , e Blood glucose ( d ) and plasma insulin ( e ) levels during an oral glucose tolerance test after 22 weeks of HFD. Corresponding area under the curve in iTBL/RβKO ( n = 8) and control ( n = 9) mice ( d , right). f , g Relative mRNA expression determined by qPCR in pancreatic islets of iTBL/RβKO and control mice of Tbl1x and Tbl1xr1 ( f ) and islet genes ( g ). Control mice Tbl1x , Tbl1xr1 , Ins1 , Ins2 , Nkx6.1 , Slc2a2 , Mafa , Pdx1 , Pax6 , Ucn3 : n = 9, control mice ChgA , Ngn3 , Ldha , Hk1 : n = 8, iTBL/RβKO mice Tbl1x , Ngn3 , Ldha , Hk1 : n = 7, iTBL/RβKO mice Tbl1xr1 , Ins1 , Ins2 , Slc2a2 , Mafa n = 6, iTBL/RβKO mice Nkx6.1 , Pdx1 , Pax6 , Ucn3 , ChgA : n = 5. h α/β-cell mass ratio of pancreatic islets from iTBL/RβKO ( n = 4) and control mice ( n = 4) on HFD, 24 weeks after knockout induction. i Representative immunofluorescent staining of insulin + (blue, β-cells) and glucagon + (red, α-cells) cells of paraffin-embedded pancreas from iTBL/RβKO and control mice on HFD, 24 weeks after knockout induction. Each point represents one mouse. Data are represented as mean ± SEM. The following statistical tests were applied: two-sided student’s t test ( d – Area under the curve, f – h ) and 2-way ANOVA with Šidák’s multiple comparison post hoc test ( b – d – time course, e ). Source data are provided as a Source Data file.

Article Snippet: The following TaqMan probes were used: Tbp - Mm01277042_m1, Tbl1x - Mm01222202_m1, Tbl1xr1 - Mm01283877_m1, Ins1 - Mm01259683_g1, Ins2 - Mm00731595_gH, Nkx6.1 - Mm00454961_m1, Slc2a2 - Mm00454961_m1, Mafa - Mm00845206_s1, Pdx1 - Mm00435565_m1, Pax6 - Mm00443081_m1, Ucn3 - Mm00453206_s1, ChgA - Mm00514341_m1, Ngn3 - Mm00437606_s1, Ldha - Mm01612132_g1, Hk1 - Mm00439344_m1, TBP - Hs00427620_m1, TBL1X - Hs00959540_m1, TBL1XR1 - Hs00226564_m1.

Techniques: Knock-Out, Control, Clinical Proteomics, Expressing, Staining, Comparison

Journal: The FASEB Journal

Article Title: A 2A adenosine receptors control pancreatic dysfunction in high-fat-diet-induced obesity

doi: 10.1096/fj.201700398R

Figure Lengend Snippet: A2AARs protect against HFD-induced β-cell dedifferentiation. mRNA expression of pdx1 (A), nkx6.1 (B), MafA (C), MafB (D), pax6 (E), sox2 (F), hes1 (G), sox9 (H), ngn3 (I), nanog (J), and oct4 (K) from pancreas of HFD-fed A2AAR-KO and WT mice. Results are representative of 3 experiments (n = 5–7 mice/group). Data are means ± sem. *P < 0.05 vs. WT littermates.

Article Snippet: The membranes were probed with polyclonal anti-cleaved PARP Ab from Cell Signaling Technology, with polyclonal anti-nkx6.1 Ab from Santa Cruz Biotechnology (Dallas TX, USA), with polyclonal anti-pdx1 Ab from Abcam (Cambridge, MA, USA) or with polyclonal anti-A 2A AR Ab from Thermo Fisher Scientific.

Techniques: Expressing

Journal: The FASEB Journal

Article Title: A 2A adenosine receptors control pancreatic dysfunction in high-fat-diet-induced obesity

doi: 10.1096/fj.201700398R

Figure Lengend Snippet: A2AARs signaling in islets preserve β-cell phenotype and GSIS in mice fed an HFD. A) GSIS of islets obtained from HFD-fed WT and A2AAR-KO mice (n = 7/group). mRNA levels of insulin (B), glucagon (C), pdx1 (D), and nkx6.1 (E) of the islets of HFD-fed WT and A2AAR-KO mice (n = 4–10/group). Western blot and densitometric analyses of protein levels of pdx1 (F) and nkx6.1 (G) from protein extract of islets obtained from WT and A2AAR-KO mice after a 16 wk HFD (n = 3/group). H) Representative images and quantitative analysis of sox2/insulin/DAPI-stained pancreas obtained from HFD-fed A2AAR-KO and WT mice after a 16 wk HFD (n = 3/group). I) mRNA expression of ARs in the islets of A2AAR-KO and WT mice (n = 4). J) Levels of A2AAR and β-actin as detected by Western blot from protein extract of pancreatic islets from A2AAR-KO and WT mice. All results are representative of 3 experiments. Data are means ± sem. *P < 0.05; **P < 0.01; ***P < 0.001 vs. WT littermates.

Article Snippet: The membranes were probed with polyclonal anti-cleaved PARP Ab from Cell Signaling Technology, with polyclonal anti-nkx6.1 Ab from Santa Cruz Biotechnology (Dallas TX, USA), with polyclonal anti-pdx1 Ab from Abcam (Cambridge, MA, USA) or with polyclonal anti-A 2A AR Ab from Thermo Fisher Scientific.

Techniques: Western Blot, Staining, Expressing